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Standard Practice for Estimation of the Spectral Bandwidth of Ultraviolet-Visible Spectrophotometers
Automaticky přeložený název:
Standardní praxe pro odhad spektrální šířky pásma Ultraviolet viditelné spektrofotometry
NORMA vydána dne 1.1.2013
Označení normy: ASTM E958-13
Poznámka: NEPLATNÁ
Datum vydání normy: 1.1.2013
Kód zboží: NS-48597
Počet stran: 10
Přibližná hmotnost: 30 g (0.07 liber)
Země: Americká technická norma
Kategorie: Technické normy ASTM
Keywords:
molecular spectroscopy, ultraviolet-visible spectrophotometers, spectral bandwidth, ICS Number Code 17.180.30 (Optical measuring instruments)
Significance and Use | ||||
4.1 These practices should be used by a person who develops an analytical method to ensure that the spectral bandwidths cited in the practice are actually the ones used. 4.2 These practices should be used
to determine whether a spectral bandwidth specified in a method can
be realized with a given spectrophotometer and thus whether the
instrument is suitable for use in this application. If accurate
absorbance measurements are to be made on compounds with sharp
absorption bands (natural half band widths of less than 15 nm) the
spectral bandwidth of the spectrometer used should be better than
1/8th of the natural half band width of
the compound’s absorption.
4.3 These practices allow the user of a spectrophotometer to estimate the actual spectral bandwidth of the instrument under a given set of conditions and to compare the result to the spectral bandwidth calculated from data given in the manufacturer's literature or indicated by the instrument. |
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1. Scope | ||||
1.1 This practice describes procedures for estimating the spectral bandwidth of a spectrophotometer in the wavelength region of 185 to 820 nm. 1.2 These practices are applicable to all modern spectrophotometer designs utilizing computer control and data handling. This includes conventional optical designs, where the sample is irradiated by monochromatic light, and ‘reverse’ optic designs coupled to photodiode arrays, where the light is separated by a polychromator after passing through the sample. For spectrophotometers that utilize servo-operated slits and maintain a constant period and a constant signal-to-noise ratio as the wavelength is automatically scanned, and/or utilize fixed slits and maintain a constant servo loop gain by automatically varying gain or dynode voltage, refer to the procedure described in 1.3 This practice does not cover the measurement of limiting spectral bandwidth, defined as the minimum spectral bandwidth achievable under optimum experimental conditions. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. |
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2. Referenced Documents | ||||
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