ASTM E3161-18

5.1 Bacteria that exist in biofilms are phenotypically different from suspended cells of the same genotype. Research has shown that biofilm bacteria are more difficult to kill than suspended bacteria 5.2 Operating the CDC Biofilm Reactor at the conditions specified in this method generates biofilm at log densities (log_{5.2.1 To achieve an S. aureus biofilm with a population comparable to that for 1.1 This practice specifies the parameters for growing a Pseudomonas aeruginosa (ATCC 15442) or 1.2 This practice uses the CDC Biofilm Reactor created by the Centers for Disease Control and Prevention 1.3 Basic microbiology training is required to perform this practice.}

1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this practice.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.