ASTM E2148-21

4.4.1.1?Test Method E1687 covers a microbiological test procedure based upon the Salmonella mutagenesis assay of Ames et al.4.4.1.2?Producers of metalworking fluids and metal removal fluids should assure themselves that virgin base oils used in the formulation of neat metalworking and metal removal oils and soluble and semi-synthetic metal removal fluids have an acceptable mutagenicity index or mutagenic potency index.

4.4.2.1?Guide E1302 defines acute animal toxicity tests and sets forth references for procedures to assess the acute toxicity of water-miscible metal removal fluids as manufactured.

4.4.2.2?Application of Guide E1302 will provide information on the acute toxicity of water-miscible metal removal fluids and will assist the user in evaluating the potential health hazards of the fluid and developing appropriate work practices.

4.4.3.1?Guide E3265 provides an overview of foaming tendency evaluation protocols and their appropriate use.

4.4.3.2?Test Methods D3519 and D3601 were withdrawn in 2013. Although each method had some utility, neither method reliably predicted in-use foaming tendency. Since Test Methods D3519 and D3601 were first adopted several more predictive test protocols have been developed. However, it is also common knowledge that no single protocol is universally suitable for predicting water-miscible metalworking fluid (MWF) foaming tendency.

4.4.3.3?There are no generally recognized reference standard fluids (either MWF or foam control additive). Instead, it is important to include a relevant reference sample in all testing.

4.4.3.4?Guide E3265 summarizes foam forming theory then provides a summary of commonly used foaming test protocols, including blender, shake, air sparge, and recirculation tests.

4.4.3.5?For each protocol, Guide E3265 explains the testing concept, apparatus needed, a summary of the test process, reporting, protocol variations, most appropriate applications and advantages, and least appropriate applications and limitations.

4.5.1.1?Practice E1497 sets forth guidelines for the safe use of metal removal fluids, additives, and biocides. This includes product selection, storage, dispensing, and maintenance.

4.5.1.2?Water-miscible metal removal fluids are typically used at high dilution and dilution rates vary widely. Additionally, there is potential for exposure to undiluted metal removal fluid as manufactured, as well as metal removal fluid additives and biocides.

4.5.1.3?Straight oils generally consist of a severely solvent-refined or hydro-treated petroleum oil, a synthetic oil, or other oils of animal or vegetable origin. Straight oils are not intended to be diluted with water prior to use. Additives are often included in straight oil formulations.

4.5.2.1?Practice E1972 sets forth guidelines for minimizing effects of aerosols in the wet metal removal environment.

4.5.2.2?Practice E1972 incorporates all practical means and mechanisms to minimize aerosol generation and to control effects of aerosols in the wet metal removal environment.

4.5.3.1?Test Method D7049 covers a procedure for the determination of both total collected particulate matter and extractable mass metal removal fluid aerosol concentrations in a range from 0.05 mg/m³ to 5 mg/m³ in workplace atmospheres.

4.5.3.2?Test Method D7049 describes a standardized means of collecting worker exposure information that can be compared to existing exposure databases, using a test method that is also more specific to metal removal fluids.

4.5.4.1?Practice E2144 covers quantitative methods for the personal sampling and determination of bacterial endotoxin concentrations in polydisperse metal removal fluid aerosols in workplace atmospheres. Users should have fundamental knowledge of microbiological techniques and endotoxin testing.

4.5.4.2?Endotoxins in metal removal fluid aerosols present potential respiratory hazards to workers who inhale them.

4.5.4.3?Users of Practice E2144 may obtain personal exposure data of endotoxin in metal removal fluid aerosols, either on a short-term or full-shift basis in workplace atmospheres.

4.5.4.4?Practice E2144 gives an estimate of the endotoxin concentration of the sampled atmosphere.

4.5.4.5?Practice E2144 seeks to minimize interlaboratory variation, but does not ensure uniformity of results.

4.5.4.6?It is anticipated that Practice E2144 will facilitate interlaboratory comparisons of airborne endotoxin data from metalworking fluid atmospheres, particularly metal removal fluid atmospheres, by providing a basis for endotoxin sampling, extraction, and analytical methods.

4.5.5.1?Practice E2169 provides recommendations for selecting antimicrobial pesticides (microbiocides) for use in water-miscible metalworking fluids (MWF). It presents information regarding regulatory requirements, as well as technical factors including target microbes, efficacy, and chemical compatibility.

4.5.5.2?Practice E2169 is not an encyclopedic compilation of all the concepts and terminology used by chemists, microbiologits, toxicologists, formulators, plant engineers, and regulatory affairs specialists involved in antimicrobial pesticide selection and application. Instead, it provides a general understanding of the selection process and its supporting considerations.

4.5.6.1?Practice E2657 covers quantitative methods for the sampling and determination of Gram-negative bacterial endotoxin concentrations in water-miscible metalworking fluids (MWF).

4.5.6.2?Users of Practice E2657 should be familiar with the handling of MWF.

4.5.6.3?Practice E2657 gives an estimate of the endotoxin concentration of the sampled MWF.

4.5.6.4?Practice E2657 seeks to minimize interlaboratory variation but does not ensure uniformity of results.

4.5.6.5?Practice E2657 is intended to relate endotoxin concentration in MWF to health effects of inhaled endotoxin.

4.5.7.1?Test Method E2563 covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.

4.5.7.2?NTM such as Mycobacterium immunogenum have been implicated as causative agents of the respiratory disease, 4.5.7.3?The measurement of viable and culturable mycobacterial densities combined with the total mycobacterial counts (including viable culturable (VC), viable non-culturable (VNC), and non-viable (NV) counts) is usually the first step in establishing any possible relationship between 4.5.7.4?Test Method E2563 can be employed in survey studies to characterize the viable culturable mycobacterial population densities of metal working fluid field samples.

4.5.7.5?Test Method E2563 is also applicable for establishing the mycobacterial resistance of metalworking fluid formulations by determining mycobacterium survival by means of plate count technique.

4.5.7.6?Test Method E2563 can be used to evaluate the relative efficacy of microbicides against Mycobacteria in metalworking fluids.

4.5.8.1?Test Method E2564 describes a direct microscopic counting method (DMC) for the enumeration of the acid-fast stained mycobacteria population in metalworking fluids. It can be used to detect levels of total mycobacteria population, including culturable as well as non-culturable (possibly dead or moribund) bacterial cells. This test method is recommended for all water-based metalworking fluids.

4.5.8.2?As noted in 4.5.7.1, non-tuberculosis mycobacteria are common members of the indigenous MWF bacterial population that have been implicated as agents of HP.

4.5.8.3?Test Method E2564 provides a quantitative assessment of the total numbers of acid-fast bacilli using acid-fast staining to selectively identify mycobacteria from other bacteria, followed by enumeration or direct microscopic counting of a known volume over a known area.

4.5.8.4?Although other microbesparticularly the Actinomycetesalso stain acid fast, they are differentiated from the mycobacteria because of their morphology and size. Non-mycobacteria, acid-fast microbes are 50 to 100 times larger than mycobacteria.

4.5.8.5?Test Method E2564 provides quantitative information on the total (culturable and non-culturable viable, and non-viable) mycobacteria populations. The results are expressed quantitatively as mycobacteria per mL of metalworking fluid sample.

4.5.8.6?The DMC method using the acid-fast staining technique is a semi-quantitative method with a relatively fast turnaround time.

4.5.8.7?The DMC method can also be employed in field survey studies to characterize the changes in total mycobacteria densities of metalworking fluid systems over a long period of time.

4.5.8.8?The sensitivity detection limit of the DMC method depends on the MF and the sample volume (direct or centrifuged, etc.) examined.

4.5.9.1?Test Method E2694 provides a protocol for capturing, extracting, and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in MWF.

4.5.9.2?Test Method E2694 measures the concentration of ATP present in the sample. ATP is a constituent of all living cells, including bacteria and fungi. Consequently, the presence of ATP is an indicator of total microbial contamination in metalworking fluids. ATP is not associated with matter of non-biological origin.

4.5.9.3?The ATP test provides rapid test results that reflect the total bioburden in the sample. It thereby reduces the delay between test initiation and data capture, from the 36 h to 48 h (or longer) required for culturable colonies to become visible, to approximately 5 min.

4.5.9.4?Although ATP data generally covary with culture data in MWF,9 different factors affect ATP concentration than those that affect culturability.

4.5.9.5?Because ATP is present in all living organisms, Test Method E2694 can be used as a first-screen to determine whether additional microbiological testing is needed.

4.5.9.6?Although there is no consensus on the exact relationship between bulk MWF bioburdens and bioaerosol concentrations, it is generally recognized that higher bulk fluid bioburdens imply higher bioaerosol concentrations.

4.5.10.1?Practice E2693 sets forth guidelines for reducing dermatitis caused by exposure to the wet metal removal environment. The scope of this practice does not include exposure to chemicals that enter the body through intact skin (cutaneous route), which has the potential to cause other toxic effects.

4.5.10.2?Practice E2693 incorporates means and mechanisms to reduce dermal exposure to the wet metal removal environment and to control factors in the wet metal removal environment that have the potential to cause dermatitis.

4.5.10.3?Practice E2693 focuses on employee exposure to the skin via contact and exposure to metal removal fluid (MRF).

4.6.1.1?Practice E2889 sets forth guidelines to control respiratory hazards in the metal removal fluid environment.

4.6.1.2?Practice E2889 adopts a systems management approach to control of respiratory hazards in the metal removal fluid environment. Elements include management practices, product selection, methods for mist minimization, machine tool design and maintenance, bioaerosol control, fluid testing and maintenance, personal protective equipment, occupational exposure guidelines, aerosol monitoring and testing methods, medical monitoring and management, and communication and training.

4.6.1.3?Practice E2889 focuses on employee exposure via inhalation of metal removal fluids and associated airborne agents. It does not include prevention of dermatitis, which is the subject of Practice E2693.

4.6.2.1?This guide collects best practices in the management of metal removal fluid systems and provides an educational tool to assist users in taking control of the MRF systems in their workplaces.

4.6.2.2?For many industrial organizations, focusing on the systematic management of MRF systems has proven effective in controlling exposures in the wet metal removal/machining environment. The recommendations are distilled from the experiences of Organization Resources Counselors member companies and represent best practice.

4.6.3.1?This criteria document reviews available information about the adverse health effects associated with occupational exposure to metalworking fluids and metalworking fluid aerosols.

4.6.3.2?Criteria documents provide the scientific basis for new occupational safety and health standards and contain a critical review of the scientific and technical information available on the prevalence of hazards, the existence of safety and health risks, and the adequacy of control methods.

4.6.4.1?This document reviews best practices as documented by the Occupational Safety and Health Administration, including engineering and work practice controls, establishing a metalworking fluid management program, instituting an exposure monitoring program, medical monitoring of exposed employees, and training.

4.6.4.2?This manual is not a standard or regulation and creates no new legal obligations. It is advisory in nature, informational in content, and is intended to assist employers in providing a safe and healthful workplace for workers exposed to metalworking fluids through effective prevention programs adapted to the needs and resources of each place of employment.