ASTM E1711-20

5.1.1?The locomotory, feeding, and social responses of fish are adaptive and essential to survival. Major changes in these responses may result in a diminished ability to survive, grow, avoid predation, or reproduce and cause significant changes in the natural population 5.2?Results from behavioral toxicity tests may be useful for measuring injury resulting from the release of hazardous materials 5.3?Behavioral responses can also be qualitatively assessed in a systematic manner during toxicity tests to discern trends in sublethal contaminant effects (5).

5.4?The assessment of locomotory, feeding, and social behaviors is useful for monitoring effluents and sediments from contaminated field sites as well as for defining no-effect concentrations in the laboratory or under controlled field conditions. Such behavioral modifications provide an index of sublethal toxicity and also indicate the potential for subsequent mortality.

5.5?Behavioral toxicity data can be used to predict the effects of exposure likely to occur in the natural environment 5.6?Results from behavioral toxicity tests might be an important consideration when assessing the hazard of materials to aquatic organisms. Such results might also be used when deriving water quality criteria for fish and aquatic invertebrate organisms.

5.7?Results from behavioral toxicity tests can be used to compare the sensitivities of different species, the relative toxicity of different chemical substances on the same organism, or the effect of various environmental variables on the toxicity of a chemical substance.

5.8?Results of behavioral toxicity tests can be useful in guiding decisions regarding the extent of remedial action needed for contaminated aquatic and terrestrial sites.

5.9?The behavioral characteristics of a particular organism need to be understood and defined before a response can be used as a measure of toxicity 5.10?Results of behavioral toxicity tests will depend on the behavioral response measured, testing conditions, water quality, species, genetic strain, life stage, health, and condition of test organisms. The behavioral response may therefore be affected by the test environment.

5.11?No numerical value or range of values has been defined as the norm for swimming, feeding, or social behavior for any fish; the detection of abnormal activity is therefore based on comparisons of the responses of exposed fish, either with activity measured during a baseline or pre-exposure period or observations of fish under a control treatment 5.12?These measures are incorporated readily into standard toxicity test protocols, with minimal stress to the test organism.