ASTM D7463-21

5.1?This test method measures the concentration of ATP present in the sample. ATP is a constituent of all living cells including bacteria and fungi. Consequently, the presence of ATP is a reliable indicator of microbial contamination in fuel systems. ATP is not associated with matter of non-biological origin.

5.2?This test method differs from Test Method D4012 as follows:

5.3?This test method does not require the use of hazardous materials and does not generate biohazard waste.

5.4?This test method can be used to estimate viable microbial biomass, to evaluate the efficacy of antimicrobial pesticides, and to monitor microbial contamination in fuel storage and distribution systems.

1.1?This test method provides a protocol for capturing, concentrating, and testing the adenosine triphosphate (ATP) present in a fuel system sub-sample (that is, test specimen) associated with:

1.2?The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens2 and a dedicated luminometer2 and reported in (instrument specific) Relative Light Units.

1.3?This test method is equally suitable for use in the laboratory or field.

1.4?Although bioluminescence is a reliable and proven technology, this method does not differentiate ATP from bacteria or fungi.

1.5?For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 ? 10¹¹ M to 3 ? 10⁸ M which is equivalent to 1 ? 10¹⁴ moles/mL to 3 ? 10¹¹ moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 ? 10¹¹ M to 2 ? 10¹⁴ M.

1.6?The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7?This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.8?This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.