ASTM E1366-11(2016)

5.1 A microcosm test is conducted to obtain information concerning toxicity or other effects of a test material on the interactions among three trophic levels (primary, secondary, and detrital) and the competitive interactions within each trophic level. As with most natural aquatic ecosystems, the microcosms depend upon algal production (primary production) to support the grazer trophic level (secondary production), which along with the microbial community are primarily responsible for the nutrient recycling necessary to sustain primary production. Microcosm initial condition includes some detritus (chitin and cellulose) and additional detritus is produced by the system. The microcosms include ecologically important processes and organisms representative of ponds and lakes, but are non-site specific.

5.2 The species used are easy to culture in the laboratory and some are routinely used for single species toxicity tests (Guide E729; Practice D3978, Guides E1192 and E1193). Presumably acute toxicity test results with some of these species would be available prior to the decision to undertake the microcosm test. If available, single species toxicity results would aid in distinguishing between indirect and direct effects.

5.3 These procedures are based mostly on previously published methods 5.4 Concurrent to measuring the ecological effects, it is advisable to measure the concentration of the parent test chemical, and if possible, the transformation products (5.5 In the microcosm, as in natural ecosystems, a population must be able to obtain its requirements from the products of other trophic levels, to maintain a birth rate equal to or greater than its death rate, and to support populations of organisms that will remove its waste products. As in natural ecosystems, several organisms might be capable of fulfilling the same function, and shifts in species dominance can occur without disruption of an ecological process. However, species that are “ecological equivalents” in one function might not be “equivalent” in other functions; for example, a filamentous alga and a single cell alga might equally produce O₂, remove NO₃, NH₃, and PO₄, but differ in the type of grazer populations they can sustain, for example, filamentous alga might support amphipods whereas unicellular algae might support 5.6 Results of these microcosm tests might be more likely to be indicative of natural ecosystem responses to chemicals than single species toxicity tests because microcosm tests can indicate the explosive population increases that might occur in a community when more sensitive competitors or predators are eliminated or the food supply is increased through competitive interactions. Also, microcosm tests are more likely to display the effects of chemical transformation or increased exposure to certain organisms by means of concentration of parent or degradation products in their food source or habitat.

5.7 A list of potential ecological effects is provided to serve as a summary (see Annex A1).

5.8 The microcosm test can also be used to obtain information on the toxicity or other effects of species or strains, not included in the control inocula (12). Additional modifications might be required.

5.9 Explicit Limitations of the Aquatic Microcosm Protocol: 

1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community.

1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.

1.4 This practice is arranged as follows:

1.5 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.