Mikrobiologie potravinového řetězce - Sekvenování celého genomu pro typizaci a genomickou charakterizaci bakterií - Obecné požadavky a návod (Norma k přímému použití jako ČSN).
NORMA vydána dne 1.12.2022
Designation standards: ČSN EN ISO 23418
Classification mark: 560057
Catalog number: 515653
Publication date standards: 1.12.2022
The number of pages: 60
Approximate weight : 180 g (0.40 lbs)
Country: Czech technical standard
Kategorie: Technické normy ČSN
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages: a) handling of bacterial cultures; b) axenic genomic DNA isolation; c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage; d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation; e) metadata capture and sequence repository deposition; f) validation of the end-to-end WGS workflow (fit for purpose for intended application). This document is applicable to bacteria isolated from: - products intended for human consumption; - products intended for animal feed; - environmental samples from food and feed handling and production areas; - samples from the primary production stage